PURIFICATION AND CHARACTERIZATION OF PEGYLATED FORM OF RECOMBINANT L-ASPARAGINASE II FROM ESCHERICHIA COLI

Author(s): S Noor Basha*, Murali K.R. Tummuru, Sudhakar Poda, V.K. Reddy Pothireddy, Narendranath Alluri

Abstract: The anti-leukemia, L-Asparagnase II (L-ASP) causes hypersensitivity reactions by forming neutralising antibodies. One of the approaches to minimise hypersensitivity of L-ASP is to modify biological and physicochemical properties without changing its activity. Thus, the present study was aimed to PEGylation of recombinant L-ASP from Escherichia coli. L-ASP was PEGylated and was purified on Q-Sepharose column. Purity of PEGylated L-Asp was found to be 100% with enzyme activity of 250± 3.4 IU. In conclusion, the PEGylation was successfully achieved with high pure enzyme.

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