IN VITRO REGENERATION OF EUPATORIUM TRIPLINERVE VAHL AN IMPORTANT MEDICINAL HERB THROUGH AXILLARY BUD CULTURE

Author(s): Gandhi K and Saravanan S

Abstract: Eupatorium triplinerve Vahl. commonly known as Ayapana belongs to the family Asteraceae is a rich source of coumarin which is widely prescribed drug warferin. The present study aimed to develop an efficient protocol for in vitro regeneration through the culture of axillary bud explant. Murashige and Skoog (MS) medium as basal medium and supplemented with different concentrations and combinations of 6-benzyl amino purine (BAP), α- naphalene acetic acid (NAA) and indole butyric acid (IBA) were employed in order to shoot induction, multiplication and rooting. The maximum percentage of shoot induction with 4.93 ± 0.06 of shoots was produced on the medium fortified with 17.76 µM/L of BAP and 2.69 µM/L of NAA. The well initiated shoots were sub cultured on fresh medium with same composition for further multiplication of shoots. The medium enriched with 22.20 µM/L of BAP and 2.69 µM/L of NAA was found to be efficient for maximum percentage of shoot multiplication with the highest number of shoots (26.00 ± 1.15) and mean shoot length of 4.00 ± 0.28 cm. The effective root induction with maximum number of roots was obtained on the half strength MS liquid medium supplemented with 9.84 µM/L of IBA. The well rooted healthy plantlets were hardened under greenhouse condition and establish successfully with 90% of survival. This protocol may be useful for the large-scale production of this plant and other medicinal plants.

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