SCREENING, OPTIMIZATION AND PARTIAL PURIFICATION OF PROTEASE ENZYME FROM DIFFERENT PULSES

Author(s): R. Subasree, S. Gayathri and Shaleesha. A Stanley*

Abstract: Enzymes are the protein molecules which are useful in detergent industry. The production of industrially important enzyme protease from different pulse varieties such as pigeon pea, green gram, Bengal gram, black chickpeas, white chickpeas, have been studied. The protease producing media for pulses have been studied. The crude enzyme is tested for protease activity by Anson method. The maximum protease activity was showed at higher rate from pigeon pea on the gelatine substrate when compared to the other samples. It was partially purified by dialysis method and molecular weight of the protein was calculated by SDS-PAGE.

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