Human Paraoxonase-1: Functional Insights Within PLGA-Based Nanoparticles (Research Article)

Author(s): R. Swathi, T. Sunitha, S. Srinu Naik, R. Redya Naik* and R. Shyam Sunder

Abstract: Human serum paraoxonase-1 (PON1) is a calcium dependent interfacial activated membrane protein associated with high density lipoproteins (HDL) play significant role in fundamental biological processes. This study determines the feasibility of formulating PON1 loaded solid lipid nanoparticles for developing a surrogate for the HDL associated PON1 in the form of PLGA (Poly Lactic glycolic acid) based solid lipid nano formulations. Studies were made on the preparation of h-PON1(human Paraoxonase-1) loaded solid lipids nano formulations with PLGA and all activities of PON-1 like lactonase, aryl esterase and paraoxonase were performed and PON1 did not lose its activity in various steps of nanoparticle preparation. Nanoparticulated PLGA based PON1 showed the highest activities.

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