DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR ESTIMATION OF METADOXINE

Author(s): P. Aravinda Reddy and Ramya Sri. S

Abstract: A rapid and precise reverse phase high performance liquid chromatographic method has been developed for the validation of Metadoxine, in its pure form as well as in tablet dosage form. Chromatography was carried out on an ODS C18 (4.6 x 150mm, 5m) column using a mixture of ACN: Water (65:35% v/v) as the mobile phase at a flow rate of 1.0ml/min, the detection was carried out at 305nm. The retention time of the Metadoxine was 3.155 ±0.02min respectively. The method produces linear responses in the concentration range of 10-50μg/ml of Metadoxine. The method precision for the determination of assay was below 2.0%RSD. The method is useful in the quality control of bulk and pharmaceutical formulations.

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