GC-MS, FT-IR and 1H NMR Profiling of Bio-Active Phytoconstituents of The TLC Fraction of Ethyl Acetate Leaf Extract of Medicinal Plant, Annona reticulata L. (Research Article)

Author(s): Subrata Mallick* and Goutam Chandra

Abstract: The aim of the present study is to identify the phytocompounds of the TLC fraction (Rf value 0.43 using solvent system of mixture of petroleum ether and ethyl acetate in 1:1 ratio) of ethyl acetate leaf extract of Annona reticulata L. through GC-MS analysis. Functional groups of the phytocompounds of the tested sample were detected by FT-IR analysis and types of protons in the phytocompounds have been confirmed through 1H NMR spectra analysis. Totally ten different bio active phytochemical compounds have been identified from mentioned TLC fraction of ethyl acetate leaf extract of Annona reticulata L. at different retention time (RT) with different percent area, and percent height through GC-MS. Several identified phytocompounds were 2-hydroxy-N,3,3-trimethylbutanamide (RT=2.5 min; % area=0.45), 2-hexanol (RT=2.85 min; % area=0.360), N-benzyl-4-hydroxypiperidine (RT=24.15 min; % area=2.17), 6,7-Dimethyl[1,2,4] triazolo [4,3-b][1,2,4] triazine (RT=29.14 min; % area=2.78), Methyl formyl (methyl) dithiocarbamate, (RT=30.95 min; % area=5.69), Butylamine N-methyl-(RT=33.11min; % area=3.03), 2-hydroxyisobutyric acid (RT=36.65 min; % area=0.61), Oxalic acid dipropylester (RT=38.25 min; % area=0.16),1,4,7,10,13 pentaoxacyclo-pentadecane (RT=38.85 min; % area=0.88), and 3,6,9,12,15-pentaonaheptadeoane-1,17-diol(RT=40.84 min; % area=2.61). The FT-IR analysis of the tested sample confirmed the presence of alcoholic OH, amine N-H, Carboxylic acid O-H, asymmetric C-H stretching, symmetric C-H stretching, CH3, ketone C=O stretching, amide C=O stretching, NH (deformation) and ring (stretching). 1H NMR spectra confirmed the presence of RCH3, ROH, R2NH, RCH2R, ArCH3, RC≡CH, R2C=CRCHR2, RCOCH2R, and RCH2OR types of protons. Identified several phytochemical compounds have the activities in pharmaceutical, agrochemical, perfume, and cosmetic industries. The presence of such phytochemical compounds may be used in traditional medicine and in agrochemical, perfume, and cosmetic industries. Some isolated pure bio active phytochemical compounds may be used for the preparation of drugs in pharmaceutical industries.

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