Evaluation of Anti-Inflammatory Activity of Aerial Part Extract of Nerium Indicum in Acute and Chronic in vivo Models in Mice

Author(s): *Nirmala Devi, Sunil Kumar Prajapati and Ajay Kumar Gupta

Abstract: Medicines from natural sources have become a mainstay in drug discovery. Biocompatibility and lower side effects of natural drugs from plants, animals, microbes and their derivatives, are the main horizons to screen such drugs. In this study aerial parts of Nerium indicum were extracted together in ethanol and distilled water. Both extract were screened for anti-inflammatory potential using three models viz., Carrageenan induced Paw edema, Cotton pellet induced Granuloma and Carrageenan induced Peritonitis. The maximum inhibition was observed for at the dose of 400 mg/kg (49.39%) and minimum inhibition at the dose of 100mg/kg (1.25%) after 4h of drug treatment in Carrageenan induced paw edema, whereas diclofenac sodium (standard drug) produced 59.03% inhibition. In the chronic model (cotton pellet induced granuloma), ethanolic extract of N.indicum (at doses of 100, 200 and 400 mg/Kg), Diclofenac as standard drug showed decreased formation of granuloma tissue by 32.84%, 39.18%, 42.07% and 67.98% respectively. In addition, exudative model of inflammation was mimicked using carrageenan induced Peritonitis. Percentage of Leukocyte and Neutrophil inhibition was calculated. Dose related inhibition was observed. At 400 mg/kg N.indicum inhibited 42.69 % leukocytes and 44.05 % Neutrophils. Thus, the results indicate that ethanolic extract of N.indicum aerial parts have potent anti-inflammatory effects on both acute and chronic type of inflammation in mice.

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