Bark Assisted Green Synthesis of Silver Nanoparticles from Walsura trifoliata (A. Juss.) Harms. (Synonym: Walsurapiscidia Roxb.) Characterization and Antimicrobial Efficacy (Research Article)

Author(s): Venkata Subbaiah Kedam*, Sujatha Karra and Savithramma Nataru

Abstract: Background: Silver nanoparticles are (AgNPs) are one of the most widely fascinating area and applicable particles whose application is enhancing in Nano world circadian. Silver nanoparticles have exposed significant applications various significant advantages give to wide range of applications in the area of bio-medical, catalysts, antimicrobials, sensors, electrons, optical fibers, bio-labeling, agriculture and other fields. Green synthesis is the safe and easiest method to produce silver nanoparticles. Due to the production of silver ions, SNPs were found to have antibacterial efficacy. Aim/ objective: The aim of the present study is the bio-synthesis of silver nanoparticles using from silver nitrate using the bark as bio-resource of the walsura trifolita (A. Juss.) Harms as cost-effective nonhazardous lessen and stabilizing admixtures. Methods: In this study silver nanoparticles prepared with adding 1mM silver nitrate to aqueous bark extract of walsura trifoliata (A. Juss.) Harms. Antibacterial activity assay with the disc diffusion method against E. coli, K. pneumonia, B. subtilis and S. aureus. Synthesized nanoparticles of AgNPs characterized by using with UV-VIS spectroscopy, DLS Zeta potential, XRD, FT-IR, SEM and TEM. Results: Produced synthesized AgNPs showed significant antibacterial activity against four bacteria i.e. E. coli, K. pneumonia, B. subtilis and S. aureus in comparison with Ag (NO3)2 solution, aqueous bark extract and Streptomycin used as standard drug. Conclusion: The secondary metabolites of Walsura trifoliata will be helpful to the phytochemists and pharmacologyts for identification of new active principle and Bio-synthesized nanoparticles is the most enlarging research area due to the biomedical application to growth novel biotechnological study.

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