Potential of Petroleum Utilization by Lysinibacillus sphaericus Isolated from Oil Contaminated Site

Author(s): Jiji Jacob and P. Prabakaran

Abstract: Bioremediation is distinctly an effective treatment in terms of efficacy, safety, cost and simplicity of administration. This study focuses on the capability of indigenous bacterial isolates on biodegradation of petroleum hydrocarbons. Soil samples of 5 different Total petroleum Hydrocarbon (TPH) sites were collected from Changanacherry district, Kerala. A total of eight oil degrading bacterial cultures were obtained and undergone morphological and biochemical characterization. Biodegradation efficiency was further studied at 4% hydrocarbon under pH7 at room temperature. Biodegraded products were analyzed by GCMS. Of them the isolate- B6 showed 94% of degradation in Gravimetric analysis and thus selected for further studies. Molecular characterization of isolate was performed and identified as Lysinibacillus sphaericus (B6). The effect of environmental factors (temperature, pH and salinity) on growth of isolate was studied and the optimum pH was 7, while that of temperature was 30° C and 1% salinity. The efficiency of degradation of petroleum hydrocarbons was 80 %. Thus, Lysinibacillus sphaericus can be used for the effective biodegradation of petroleum hydrocarbons.

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