Green Synthesis, Characterization, and Antimicrobial Activity of Silver Nanoparticles with Tuber Extract of Cyanotis tuberosa (Roxb) Schult. and Schult F.

Author(s): Gorantla Ramakrishna and *Nimmanapalli Yasodamma

Abstract: Plant mediated synthesis of nanoparticles has wide application in biomedicine due to its novel properties and its eco-friendly nature. The present study deals with the biosynthesis of stable silver nanoparticles (SNPs) from the tuber aqueous extract of Cyanotis tuberosa. The synthesized nanoparticles are characterized by the colour change, observed from gray to brown indicates the formation of nanoparticles and UV–VIS surface plasmonresonance spectroscopy observed at 427 nm further confirmed the synthesized nanoparticles as SNPs.FTIR spectroscopic studies confirm that phenols, amines and halides of tuber extract is mainly responsible for capping and stabilization of synthesized SNPs. The XRD data shows crystalline nature of nanoparticles and EDAX measurements reveals the presence of 30.94% Ag metal. Zeta potential at -18.6mV the negative value indicates the high stability of nanoparticles. TEM microscopic analysis revealed that the size of synthesized SNPs ranging from 16 to 35 nm with spherical shape. Further, the antibacterial studies of synthesized SNPs show high activity towards Klebsiella pneumonia with 21mm diameter zone of inhibition followed by staphylococcus aureus, Eschirichia coli and Bacillus subtilis.

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