Phytochemical Screening of Hibiscus tiliaceus by FTIR Spectroscopic Analysis

Author(s): Rawool Prachiti P and Parulekar Berde C*

Abstract: Different parts of Hibiscus tiliaceus plant such as leaves, bark, flower and roots have medicinal properties and thus H. tiliaceus is potentially allelopathic and may produce compounds that are useful in Ayurveda. So to determine presence of various phytochemicals from the plant extracts, a simple, precise Preparative Thin layer chromatography was performed. Initially to confirm the presence of phytochemicals, qualitative tests for Tannins, Phlobatannins, Saponins, Flavonoids, alkaloids, Quinones, Coumarine, Triterpenoids, Cardi-glycosides, Anthraquinoacne glycosides, Steroids, Phytosterols were performed. The methanolic extract of leaves, bark, flower and root of H.tiliaceus upon separation using preparative TLC, showed separated bands. In this study, the most suitable solvent system for analysis was found to be chloroform: glacial acetic acid: methanol: water in the ratio 6:2:1:1 with the highest resolution power. Separated TLC bands, after purification were further analysed for their antimicrobial activity against pathogens by using agar cup method. Air dried samples were subjected to FTIR analysis. FTIR spectrum confirmed the presence of alcohols, phenols, alkanes, alkynes, alkyl halides, aldehydes, carboxylic acids, aromatics, nitro compounds and amines in different extract.

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