Phytochemical Analysis and FT-IR Fingerprinting of Pineapple Peel-A Natural Resource of Bioactive Compounds

Author(s): A.S. Lubaina*, P.R. Renjith and K.V. Dinesh Babu

Abstract: Aims: Fruit wastes and their by-products are formed in great amounts during industrial processing and its disposal being one of the major problems faced by all parts of our state. The present study is an attempt to recycle pineapple peel - a byproduct of pineapple processing industries to study the qualitative and quantitative analysis of phytochemicals and FT- IR reflectance spectra of the petroleum ether, ethyl acetate, ethanol and aqueous extracts. Materials and methods: The four solvent extracts of pineapple peel were qualitatively screened for the presence of different phytochemicals followed by the quantification of phenols and flavonoids. Tablets for FT-IR spectroscopy were prepared in an agate mortars, by mixing extract powder with KBr and the absorbance spectra were measured between 400 and 4000/cm. Results: The qualitative phytochemical screening of the petroleum ether, ethyl acetate, ethanol and aqueous extracts of pineapple peel revealed the presence of phenols, flavonoids, alkaloids, tannins, saponins, terpenoids and steroids. The estimation of the total phenols and flavonoids showed an enhanced profile of phenols compared to flavonoids. The FT-IR spectrum exhibits the characteristic fingerprint banding features in all the extracts. Several indicator bands that are pertained to functional groups represent chemical components or metabolic products. Among the four extracts evaluated the ethyl acetate fraction showed significant amount of phytochemicals as well as IR fingerprinting pattern. Conclusion: The pineapple peel contained an appreciable amount of phytochemicals and bioactive compounds revealed it’s potential for the utilization of pineapple waste in pharmaceutical as well as cosmetic industries.

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