Phenol Biodegradation by Bacillus aryabhattai k13 From Paper Mill Effluent Under Optimized Conditions

Author(s): Viddamuri Swetha and Edla Sujatha

Abstract: The environment has been largely contaminated by variety of toxic compounds. Especially through discharge of industrial effluents. Phenol is an organic compound extremely toxic to microorganisms, plants, fish and animals. Therefore, it is necessary to eliminate from the environment. The present investigation is aimed to isolate phenol degrading bacteria, its characterization and treatment of paper mill effluent with selected bacteria Bacillus aryabhattai k 13 under optimized conditions of pH, temperature, and concentration of carbon and nitrogen sources, shaking speed. The isolated bacterial strain from paper mill effluent was found to be excellent for phenol degradation. The isolate B.aryabhattai from paper mill effluent found to utilize phenol as a source of carbon and energy. The optimized pH for phenol degradation was neutral and temperature of 35⁰C -37⁰C at 48-96hrs. The culture medium supplied with 0.5 gm/L of urea has stimulated the phenol degradation. Maltose at concentration of 1.0 gm/L was more suitable for phenol degradation. The bacterial strain was able to degrade phenol concentration of1200mg/L at 144hrs of incubation under 150rpm shaking condition. The results indicate that isolate Bacillus aryabhattai has much potentiality strain to use in bioremediation of phenol contaminated soils.

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