Analytical Method Development and Validation of Pyrimethamine and Sulphadoxine in Pharmaceutical Dosage Forms by RP-HPLC

Author(s): Venkata Rao Vutla, Bhadru Banothu and Vidyadhara Suryadevara

Abstract: A simple, accurate reverse phase high performance liquid chromatographic method has been developed for the simultaneous estimation of Pyrimethamine and Sulphadoxine in bulk and Pharmaceutical dosage forms. The analytical method development was carried on Agilent made HPLC instrument using RP - C18 column. The mobile phase employed for the estimation is Phosphate Buffer: Acetonitrile which was pumped at a flow rate of 0.9 mL min-1 in the ratio of 70:30v/ v and the eluents were monitored at 224 nm. Linearity was obtained in the concentration range of 5-37.5 μg/ml for Pyrimethamine and 100-750 μg/ml for Sulphadoxine. The correlation coefficient and % curve fitting for Pyrimethamine and Sulphadoxine was found to be 0.9997 and 0.9999 respectively. The mean percentage recovery for Pyrimethamine and Sulphadoxine was found to be between 99.51-100.53% and 99.44-100.46% respectively. The method was statistically validated and RSD was found to be less than 2% indicating high degree of accuracy and precision of the proposed HPLC method. Due to its simplicity, rapidness, high precision and accuracy, the proposed HPLC method can be applied for determining Pyrimethamine and Sulphadoxine in bulk and in pharmaceutical dosage form.

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