In-silico Characterization of Translated Protein Obtained from the Crab Dromia Dehaani (Rathbun, 1923)

Author(s): Wilson Sylvester Fredrick* Velayudham Sadeesh Kumar and Samuthirapandian Ravichandran

Abstract: Crustaceans play an important role in major fisheries. The non edible crab Dromia dehaani which are collected from the trash, was chosen for the study as it has the high protein estimation and molecular weight among the four crabs. The molecular characterisation was identified by 18s RNA sequence. In this present study, the nucleotide sequences of crab D.dehaani is translated to protein sequences through EMBOSS software in order to know the primary structure analysis, physicochemical properties and secondary structural states that the protein involved is a membrane protein with 2 transmembrane helices and it is in hydrophobic in nature. The physicochemical properties were analysed by Protparam tool. The secondary primary structure was predicted by SOPM and SOPMA. The SOSUI server performed the identification of transmembrane regions in proteins. The tool BioEdit was used to compute the Kyte and Dolittle mean hydrophobicity profile of the transmembrane regions. Multiple sequence alignment of transmembrane regions computed using MSA tool was used to generate the sequence logo of transmembrane regions. Therefore, the computational tools are helpful in understanding the essential biological processes involved in conserved sequence specific interaction between DNA/RNA and the proteins.

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