Phytochemical Analysis of Ruellia tuberosa Tuber Ethanolic Extract Using UV-VIS, FTIR and GC-MS Techniques

Author(s): M.N.L.C. Harika and P. Radhika

Abstract: Aim: The aim of this study was to evaluate various bioactive compounds present in the roots of Ruellia tuberosa collected from the biodiversity park located at Visakhapatnam. The study of ethanolic isolates based on characterization using gas chromatography- Mass spectroscopy. Materials and Methods: Preliminary phytochemical analysis showed the presence of alkaloids, phenolics, flavonoids, steroids, terpenoids, coumarins, tannins, cardiac glycosides, carbohydrates and amino acids. Further investigation was done using Ultraviolet-Visible spectroscopy, Fourier transform infrared spectroscopy (FTIR) and GC-MS analysis. Results: UV-VIS and IR spectral analysis showed the peaks of corresponding functional groups present in the phytochemical constituents. GC-MS analysis of R. tuberosa tuber shows total sixteen compounds from its ethanolic extract. Out of these compounds, the highest abundance of them found to be Flavone (50%), E, E, Z-1, 3, 12-nonadecatriene 5, 14-diol (41.8%), Phytol (41.4) and Methyl-6-octadecenoate (40.2) present, based on the highest percentage of peak area. Conclusion: The present study demonstrated that Ruellia tuberosa tuber has rich source of secondary metabolites.

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