Effect of Flavonoids on Cell Viability and Glutamate-Induced Migration of U87MG Glioma Cells

Author(s): Arpana Sharma, J.P.N Mishra and Rana. P. Singh

Abstract: Aim: To evaluate anticancer effect of the flavonoids (fisetin, acacetin and silibinin) on glioma cells. Methods: U87MG cells, glioblastoma grade IV tumor, were used as the invitro model to assess the anticancer effects of phytochemicals fisetin, acacetin and silibinin. Cell viability assay was performed by MTT. Cell growth and death assay was checked by trypan blue dye assay. Wound healing assay was used to check the migration of the cells. RT-PCR, western and gelatin zymography were performed for MMPs expression. Results: We found that number of live cells and cell proliferation were decreased by all three flavonoids and fisetin showed better effects as compared to acacetin and silibinin as performed by MTT and trypan blue assays. Fisetin significantly decreased migration more as compared to acacetin and silibinin as observed in the wound healing assay. Furthermore, we found glutamate promoted cell migration which was inhibited by fisetin in U87MG cells. We found that MMP-2 and MMP-9 expression levels decreased in the presence of fisetin as performed by zymography, RT-PCR and immunoblotting. Conclusion: Fisetin significantly decreases cell growth, proliferation and migration and the effects were relatively better as compared to acacetin and silibinin. Fisetin inhibits PCNA, MMP-2 and MMP-9 expression. All these results suggested that fisetin had anticancer capability through influencing the cell viability and migration of U87MG cells.

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