Wound Healing Mechanism by the Standardized Extracts of Acalypha indica and Aristolochia bracteolata on Human Cell Lines

Author(s): Preethi Jenifer, Mangathayaru Kalachaveedu* and Murugan Girija Dinesh

Abstract: Acalypha indica and Aristolochia bracteolata are two traditional herbal medicines used for treatment of various diseases particularly for skin disorders. They are being used in Siddha and Ayurvedic treatments. To investigate the action mechanism on wound healing process, the in vitro wound healing assays were carried out using Human dermal fibroblasts and Human epidermal keratinocytes. The aerial parts of Acalypha indica and Aristolochia bracteolata were extracted using ethanol and evaluated for its efficacy on viability and migration by MTT and Scratch wound assay. The genes associated with its wound healing property were evaluated by RT-PCR and angiogenic activity by CAM assay. Proliferative capability of the extracts was confirmed by MTT assay. Results showed that the wound healing mechanism involves the activation of wound healing related genes of MMP-2, Collagen type 1 and Collagen type 4 in HDF cells. The expression of MMP-2 facilitates essential events of wound migration, angiogenesis and tissue remodelling. Collagen 1 and 4 structures the ECM matrix during the tissue repair. The construction of new blood vessels (angiogenesis) by the plant extracts helps in supplying nutrients necessary for the cell proliferation and migrations.

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