Comparative Analysis of Extracted and Exfoliated Deciduous Tooth for Mesenchymal Progenitor Cell Banking

Author(s): Ranjith Kumar Indarapu and Pavana Jyothi Cherukuri*

Abstract: Human deciduous teeth have the ability to produce multipotent mesenchymal progenitor cells. Mesenchymal Progenitor cell (MPC’s) applications are increasing ever in regenerative medicine & tissue engineering. In this present study focused on comparative analysis of the banking feasibility between mesenchymal progenitors cells cultured from extracted versus exfoliated deciduous tooth. Mesenchymal progenitor cells harvested from both extracted and exfoliated tooth samples were analyzed for their proliferation rate, progenitor cell surface markers expression, multilineage trans-differentiation potential and colony forming unit assay. Progenitor cells from the extracted tooth and exfoliated tooth sample have shown similar characteristics in morphology, cell surface marker expression and differentiation potentials. Exfoliation while is a natural relatively painless process, extraction involves sedating the person and extracting the tooth via invasive procedures, thereby causing discomfort to the patient. Results obtained by this study recommend that isolation and harvesting of MPC’s from dental source by exfoliated method is more feasible than extracted method.

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