Production of Laccase from Fungus Pleurotus florida Cultured in Different Agro-Wastes

Author(s): P. Muthupriya*, N.R. Minu Bharathy and K. Siva Kumar

Abstract: The effluent of agriculture based industries is recyclable and could be converted to useful output. The main components of agro-wastes are lignin and cellulose which are not easily degraded. Microorganisms like bacteria and fungi secrete the enzymes which are able to oxidize such components. In the present study, optimization of the growth condition for the production of lignin degrading enzyme laccase by fungus Pleurotus florida using agrowaste such as baggase, orange peel, sweet lime peel, banana peel and grape stalk as substrates and its partial purification was undertaken. The fungus P. florida produces highest laccase activity (0.42 U/ml/min) using sweet lime peel than other substrates. Solid state fermentation for laccase production was optimised at 96 hrs fermentation time, 5 ml of inoculum volume and 60 % moisture content. The enzyme laccase produced by P. florida using sweet lime peel was investigated for the effect of nitrogen sources and inducers. Among different nitrogen sources tested, soy peptone showed highest laccase activity, similarly guaiacol increased laccase activity up to 0.48 U/ml/min. The enzyme laccase was partially purified with 60% ammonium sulphate and showed single subunit when it was subjected to SDS-PAGE with molecular a weight of 66kDa.

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