EFFECT OF MUTAGEN ON FREQUENCY AND SPECTRUM OF CHLOROPHYLL MUTATION IN CHICKPEA (CICER ARIETINUM L.) THROUGH INDUCED MUTATION

Author(s): Manisha G. Nagargoje and Navnath G. Kashid

Abstract: Mutation breeding can constitute a valuable tool to the conventional breeding methods in widening the genetic base of cultivated germplasm in crops through creation of some useful mutants, henceforth, mutation breeding finds a prominent place in the augmentation and recreation of genetic variability and has played a significant role in the development of many crop varieties. The frequency of chlorophyll mutation is useful in assessing the potency of mutagen and it is also an indicator of factor mutation. It is considered as a dependable index for evaluating genetic effects of mutagenic treatments. Chlorophyll mutation helps in the study of the effects of specific genes products in differentiations. Mutations in genes responsible for chlorophyll synthesis bring outs deficiency of chlorophyll pigments. The mutagenic effectiveness is reflected in the segregation of chlorophyll mutants and it is also a good indicator to forecast the spectrum of genetic variability that can arise from the mutated sectors. In present investigation a wide spectrum of chlorophyll mutations like albina, xantha, chlorina and viridis could be recorded in M2 generation, grown from the harvested seeds of M1 in Chickpea. The frequency of these mutants revealed an increasing trend with an increasing concentration of all the mutagens in both the cultivars of chickpea. The frequency and spectrum of chlorophyll mutants induced by EMS, SA and Gamma rays’ mutagens in Chickpea varieties BDNG-797 and PG 0408. High frequency of mutant in BDNG 797 were recorded in SA 0.04% treatment while in PG0408 highest frequency was observed while 0.06% SA treatments.

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