IN VITRO SCREENING OF THE PROBIOTIC POTENTIAL OF LACTOBACILLUS AND ENTEROCOCCUS STRAINS ISOLATED FROM HUMAN BREAST MILK, FECES OF BREAST-FED INFANTS AND ANIMAL MILK (GOAT, COW AND BUFFALO)

Author(s): Shaik Parvinnisa, Farhath Khanum*, Chandrasekhar. N, Girish Kumar.B and Renuka.B

Abstract: This study sought to investigate the probiotic potent of lactic acid bacteria isolated from animal raw milk, human breast milk and infant fecal matter. A total of 200 LAB strains have been isolated, among this four Lactobacillus spp and four Enterococcus spp. have been screened for their functional properties, among these Lactobacillus para casei NB16 isolated from human breast milk was capable to survive at 1% bile salt, pH 2.0 and SGJ for 4 h without losing viability and ability to grow in a range of temperatures at15- 50oC, pH 3-9 and salt concentration up to 8 %. All LAB strains exhibited inhibitory activity towards wide range of food borne pathogens, in addition, NB12, and NB16 have been found to be resistant to 16 antibiotics out of 17 except Chloramphenicol and fermented 17 sugars out 20. Adhesion percentage of 8 isolates to Hydrocarbons up to (96%), auto-aggregation up to (90%) and co-aggregation with Escherichia coli MTCC 40 up to (62%) was observed and 16S rDNA sequence confirmed NB12, NB 14, NB 113 as Lactobacillus para casei, NB16 as Lactobacillus casei, NB10, NB44, NB94 as Enterococcus faecium and NB7 as Enterococcus faecalis respectively. Probiotic functional properties of isolates have been characterized and isolates were identified by using molecular methods.

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