THE STUDIES ON UNDERSTANDING OF MIDGUT BACTERIAL COMMUNITY OF AEDES AEGYPTI COLLECTED FROM TAMILNADU, INDIA- A METAGENOMIC APPROACH

Author(s): Suganya K., *Rajesh Sing J., Ganesh P., Pinku Satnami and Stalin Antony

Abstract: Metagenomics as a new field of research has been developed over the past decade to elucidate the genomes of the non-cultured microbes with the goal to better understand global microbial ecology on the one side, and on the other side it has been driven by the increasing biotechnological demands for novel enzymes and biomolecules. Ingestion of blood meal by female mosquitos triggers a series of physiological processes in the midgut and also exposes them to infection by these pathogens. The bacteria normally harbored in the mosquito’s midgut are known to influence physiology and can also alter the response to various pathogens. Here, we studied the bacterial diversity found in midgut part of Aedes aegypti to understand the host and microbe interaction. The adult Aedes aegypti mosquitos were collected from Chidambaram and around area using ovitrops and midgut part was extracted moreover the DNA templates were isolated and amplified by PCR. The DNA amplicons were sequenced by Illumina MiSeq gene sequence. The total 179,310 reads were classified in to 209, the bacterial genera of Aeromonas, Klebsella, Acinetobacter, Staphylococcus, Bacillus and Pseudomonas predominantly found to be high when compared to the other bacterial genera. The present data strongly encourage further investigations to verify the potential role of the detected bacteria in mosquito for the transmission several vectoral diseases.

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