DEVELOPMENT AND VALIDATION OF UV-SPECTROPHOTOMETRIC METHOD FOR ESTIMATION OF PICROSIDE-II IN PICRORHIZA KURROA RHIZHOME EXTRACTS

Author(s): Sachin Bhusari*, Avinash Chaudhary and Mahesh Rindhe

Abstract: Aim: To develop and validate a simple, precise and cost-effective UV- visible spectrophotometric method for the estimation of Picroside II in Picrorhiza kurroa rhizome extracts according to the ICH Q2 (R1) guideline. Methods: Spiked Picroside-II solution was scanned over UV-visible range for its wavelength of maximum absorbance. Various calibration standards of Picroside II were prepared. Calibration curve of concentration vs. absorbance was plotted. Various analytical method validation parameters were calculated. Results: The maximum wavelength of Picroside II was found to be 266 nm. The correlation coefficient at concentration range of 20-140 μg/ml was found to be 0.9995. The validation of the developed UV method was carried out by conducting linearity, accuracy, precision, robustness, ruggedness, limit of detection and limit of quantitation studies. Developed UV method was found to be precise for the intra-day and inter-day study and shows percent relative standard deviation in the range of 0.1179 to 1.3001 & 0.241 to 0.8882 respectively. The total percent recovery of Picroside II was found to be 99.85 to 99.85 %. Conclusion: A simple, precise and cost-effective UV- visible spectrometry method for the estimation of Picroside II in standardized extract of rhizomes of Picrorhiza kurroa was developed. The said method was developed using solvent containing economical percentage of organic phase in aqueous media. Said validated UV- visible method can be efficiently used for the estimation of Picroside II in extracts of rhizomes of Picrorhiza kurroa.

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