OPTIMIZATION OF CARBOXYMETHYL CELLULASE PRODUCTION FROM HALOMONAS SP. ISOLATED FROM SALTPAN

Author(s): N. Benit, G. D. Biji and P. Vijayaraghavan

Abstract: Cellulases are glycoside hydrolases involved in the depolymerization of cellulosic materials and have wide applications in industries, such as in the textile, food and feed industry. In addition, supplementation of these enzymes with animal feed improves the digestibility of plant materials. The important application is the production of bioethanol, as a result of search of alternate sources for energy needs. In this study, carboxy methyl cellulase (CMCase) producing Halomonas sp. was isolated from saltpan. The culture conditions like temperature, pH, carbon source and nitrogen source were optimized. The optimum conditions for CMCase production were 35 °C at pH 9.0 with glucose as carbon source and yeast extract as nitrogen source. Halomonas sp. PV1 showed good CMCase activity and may be used in various industrial applications.

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