IN VITRO STABLE PLANTLET REGENERATION FROM CALLUS CULTURE OF CICER ARIETINUM L.

Author(s): Samanta D, Bhowal P, Chakraborty N Ghosh J and Roy D*

Abstract: A rapid, simple and efficient protocol for plantlet regeneration was achieved through organogenic callus from internode explants of Cicer arietinum. Callus induction and organogenesis were significantly dependent on the presence/ absence and type of growth regulators. Internodes showed higher growth potential compared to leaf and node for callus induction. Callus induction was obtained after 15 days of inoculation in MS medium supplemented with 2,4-D 1mg/l. The highest shoot formation was obtained when BAP 1mg/l was used. The best response for rooting was obtained in 2,4-D 0.5 mg/l. Cytological study indicated that the chromosome number remain same (2n= 16 chromosomes) in callus and in vivo roots. Callus cytology revealed that callus tissues were diploid in nature. Karyotype analysis indicated that the number and type of both primary and secondary constrictions remain same in callus and in vivo roots. Thus, this can be an easier protocol for stable plant regeneration in Cicer arietinum. Rooted plantlets were successfully hardened under culture conditions and subsequently established in the field conditions. The recorded survival rate of the plants was 67.3%. Plants were without any detectable phenotypic variations.

PAGES: 648-655  |  43 VIEWS  193 DOWNLOADS