CHLOROFORM FRACTION OF PARKIA JAVANICA BARK IS THE MOST POTENT SOLVENT FRACTION REGARDING ANTIBACTERIAL ACTIVITY AGAINST STANDARD BACTERIAL SPECIES COMMONLY FOUND IS SKIN WOUND

Author(s): Saha S, karmakar P and Sil S K

Abstract: Aim: To evaluate potent solvent fraction of Parkia javanica having antibacterial activity against standard bacterial strains which are commonly found in skin wound. Methods: The different solvent fractions of Parkia javanica were screened for antibacterial activity against bacterial species predominantly found in chronic wound, by serial dilution technique. Growth kinetics study was performed and percentage of ROS production was measured by NBT reduction assay and finally reporter gene assay was performed to understand the mode of action of the active fraction of this study plant. Results: The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were obtained with a range of IC100 dose of 0.1563 to 0.625 mg/ml in case of standard bacterial strains. Chloroform fraction of Parkia javanica was comparatively more potent than the other fractions. The lag phase of all treated bacteria is extended compared to untreated cells. The normalized % of ROS is increased in presence of chloroform fraction and this fraction is also responsible for breakdown of the plasmid DNA. Conclusion: This study suggests that, the chloroform fraction of Parkia javanica possesses promising antimicrobial substances which are having activity against Standard ATCC bacterial species and ROS induced DNA damage could be the possible mediator of its antimicrobial activity.

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