ANTIFUNGAL SCREENING OF ANNONA SQUAMOSA AND SPONDIAS PINNATA AQUEOUS LEAF EXTRACT ON 3 FUNGAL GENERA ISOLATED AND IDENTIFIED FROM ORYZA SATIVA ROOT AND SEED

Author(s): Chinju Merin Abraham* and Soumya Prasad

Abstract: Through the study, we isolated and identified five pathogenic fungal genera from Oryza sativa roots and seeds which cause serious ill-effects to life. These were identified as Rhizopus, Aspergillus, Cylindrocarpon, Penicillium and Curvularia. Preliminary phytochemical screening of aqueous leaf extracts of Annona squamosa and Spondias pinnata were conducted and their actions as antifungal agent on 3 fungal genera were determined by disc diffusion method. Among the screened phytochemicals, Spondias pinnata shows maximum types of secondary metabolites in aqueous extract. Cylindrocarpon, Penicillium and Curvularia were used as test organism. The extracts having antimicrobial activity has varied diameter of inhibition zone among tested organisms. Spondias pinnata leaf has antifungal activity against Cylindrocarpon, Penicillium and Curvularia with zone of inhibition of 12mm, 11mm and 5mm respectively. Annona squamosa has antifungal activity against Penicillium and Curvularia with zone of inhibition of 8mm and 5mm and no markable activity against Cylindrocarpon. The highest level of antifungal activity was recorded in Spondias pinnata which was active against all tested organisms that may be due to the different types of phytochemicals.

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